Pontibacter beigongshangensis sp. nov., Isolated from the Mash of Wine

A Gram-negative, aerobic, oval-shaped, and light red pigmented bacterium, designated T6-1 T , was isolated from the mash of wine collected from a wine-making laboratory simulated fermenter located in Beijing, China. The optimal growth of T6-1 T occurred at 30 °C, pH 7.0 with 1% NaCl. The sole respiratory quinone was menaquinone-7 (MK-7). The principal cellular fatty acids (>5%) were iso-C 15:0 , iso-C 17:0 3OH, C 16:1 ω5c, and iso-C 17:0 . The major polar lipids were PE (phosphatidylethanolamine), PL (unidentified phospholipid), and L1-2 (unidentified lipids). 16S rRNA phylogenetic analysis indicated that strain T6-1 T belonged to the genus Pontibacter . The 16S rRNA gene sequence of strain T6-1 T was most similar to Pontibacter amylolyticus 9-2 T (95.92%). The genomic DNA G+C content of strain T6-1 was 50.34 mol%. The digital DNA–DNA relatedness and average nucleotide identity value between T6-1 T and 9-2 T was 20.20% and 74.18%, respectively. Polyphasic taxonomy analysis indicated that strain T6-1 T represents a novel species of the genus Pontibacter , for which the name Pontibacter beigongshangensis sp. nov. is proposed, with the type strain T6-1 T (= CGMCC 1.17104 T = KCTC 72413 T ).