P1.01-015 Polyphenols-Rich Fruit Extracts Prevent Tobacco Specific Nitrosamine-Induced DNA Damage in Lung Epithelial Cells: Topic: Protective Factors, Risk Reduction, Smoking Cessation

Diets rich in polyphenols are well-known to reduce lung cancer risk among high-risk populations. We analyzed the efficacy of polyphenols-rich Haskap (Lonicera caerulea L.) fruit extracts in preventing tobacco specific nitrosamine (TSNA)-induced DNA damage in BEAS-2B lung epithelial cells. Monomeric polyphenols of Haskap fruits were extracted in ethanol and water, and profiled. TSNA, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and 4-[(acetoxymethyl) nitrosamino]-1-(3-pyridyl)-1-butanone (NNKOAc) were used (at sub-lethal concentrations) independently to induce the carcinogenesis process in BEAS-2B cells. Cell viability assay was confirmed that the tested concentrations of Haskap extracts were not cytotoxic to BEAS-2B cells. The Haskap extracts contain diversity of polyphenols including phenolic acids and flavonoids, however, cyanidin-3-O-glucoside was the most predominant. Pre-treatment of cells with the Haskap extracts could significantly reduce the NNK- and NNKOAc-induced DNA double strand breaks, DNA fragmentation and intracellular reactive oxygen species, compared to non-treated cells. Immunocytochemistry for H2AX-phosphorylation (Serine 139, red) A. NNKOAc 100 μM, 3 h; B. Haskap ethanol extract (50 μg/mL, 3 h)+NNKOAc (100 μM, 3 h). DNA counter-staining was performed with 4,6-diamino-2-phenylindole (blue). The polyphenols-rich Haskap extracts could prevent TSNA-induced DNA damage in lung epithelial cells in vitro. Protective effects of Haskap polyphenols against DNA damage are being investigated in vivo using A/J mice.