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Baculovirus Variant Detection from Transient CRISPR-Cas9-Mediated Disruption of Gp64 at Different Gene Locations

Madhuja Chakraborty, Lisa Nielsen,Delaney Nash, Mark R. Bruder,Jozef I. Nissimov,Trevor C. Charles,Marc G. Aucoin

International Journal of Molecular Sciences(2025)

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Key words
baculovirus,CRISPR-Cas9,tiled-amplicon sequencing,bioinformatics pipeline,variant calling pipeline,transfection-infection assay (T-I assay),whole genome sequencing,next-generation sequencing,p6.9 promoter,AcMNPV
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