Uncovering Synaptic and Cellular Nanoarchitecture of Brain Tissue Via Seamless in Situ Trimming and Milling for Cryo-Electron Tomography

Jiying Ning,Jill R Glausier,Rangana Warshamanage, Leslie Gunther-Cummins, Tom Burnley, Colin M Palmer,Guillermo Gonzalez-Burgos,Takeaki Miyamae, Jing Wang, Diane Carlisle,Chyongere Hsieh, Thomas Schmelzer, Silas A Buck, Jonathan Franks,Cheri M Hampton,William R Stauffer,David A Lewis,Robert M Friedlander, Frank P Macaluso,Martyn Winn, Michael Marko,Zachary Freyberg

bioRxiv the preprint server for biology（2025）

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