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Multi-stage-mixing to Create a Core-then-shell Structure Improves DNA-loaded Lipid Nanoparticles Transfection by Orders of Magnitude

Jia Nong, Xijing Gong, Quang Minh Dang,Sachchidanand Tiwari, Manthan Patel,Jichuan Wu, Andrew Hanna, Wook-Jin Park,Elena N Atochina-Vasserman, Hui-Ting Huang,Oscar A Marcos-Contreras,Kahlilia C Morris-Blanco, Jonathan J Miner,Drew Weissman,Vladimir R Muzykantov,Kushol Gupta,David Issadore,Jacob W Myerson,Zhicheng Wang,Jacob S Brenner

bioRxiv the preprint server for biology（2025）

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