Homemade Isothermal Amplification-Initiated Cas14a Assay for Rapid Quantitative Detection of Aquatic RNA Virus Gene with No PAM

Xiufen Yang, Lin Lu, Yanchi Luo,Qingwei Wang, Jianing Wang, Yihua Ren, Yuwei Wu,Masoud Negahdary, Khaydar E. Yunusov, Sherzod A. Yuldoshov, Abdushkur A. Sarimsakov, Haimei Mao,Yi Wan,Fengge Song

AQUACULTURE（2025）

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Red-spotted grouper nervous necrosis virus,Protospacer adjacent motif-free,CRISPR-Cas14a,RT-LAMP

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