Protocol for continuous video-EEG/EMG recording to study brain function in neonatal rats

The electroencephalogram (EEG) is crucial for real-time brain physiology research in epilepsy. However, maternal care reliance limits its use in immature rodents. Our “pup-in-cup” setup overcomes this, enabling continuous, uninterrupted video-EEG/electromyogram (EMG) recordings in neonatal rats. This protocol details the steps for video-EEG/EMG system setup, EEG headmount implantation, and recording continuous video-EEG/EMG traces from postnatal days 4–12.For complete details on the use and execution of this protocol, please refer to Choudhary et al.1