Quality evaluation of Bazhen Yimu Pill based on three-wavelength fusion fingerprint and electrochemical fingerprint combined with antioxidants

Bazhen Yimu Pill (BZYMP) is a concoction of 9 Chinese herbs with pharmacological effects such as vasodilatation, inhibition of platelet aggregation and uterine stimulation. Due to its complex composition and the interaction of multiple chemical components, it presents challenges in quality control. The aim of this study was to construct a fingerprint profile of BAYMP and to conduct a study on the relationship between the fingerprint profile and therapeutic effects. Firstly, 2 fingerprint profiles of BZYMP were established using high performance liquid chromatography (HPLC) and electrochemical analysis. In addition, in order to avoid the incomplete chemical information extracted from a single wavelength, a three-wavelength fusion fingerprint (TWFP) was established and a clear and easy-to-understand electrochemical fingerprint (ECFP) was innovatively established by using electrochemical analysis, and 9 characteristic parameters were recorded. Subsequently, the quality consistency of BZYMP was qualitatively and quantitatively evaluated by the comprehensive linear quantitative fingerprint method (CLQFM) method, while the fingerprint efficacy relationship between the fingerprint peaks of BZYMP and the IC50 value of the DPPH assay was constructed by the OPLS model, and the relationship between the fingerprint peaks and the electrochemical analyses was explored by using the Pearson correlation coefficient. This study examined BZYMP from two dimensions, quality consistency and efficacy, and provided a new idea for other traditional Chinese medicines and their preparations to realize the dual control of quality and efficacy.