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Content of Nitrogen Monoxide and Copper in the Hippocampus of a Rat Model of Short-Term Cerebral Ischemia Followed by Reperfusion

Kh. L. Gainutdinov,V. V. Andrianov,G. G. Yafarova,L. V. Bazan,T. K. Bogodvid,V. S. Iyudin, T. A. Filipovich, Yu. G. Shanko,Yu. P. Tokalchik,V. A. Kulchitsky

Biophysics（2024）

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Abstract
Electron paramagnetic resonance (EPR) spectroscopy is used to determine the content of nitric oxide (NO) and copper in the hippocampus of healthy rats and rats under ischemia modeling. Ischemia is modeled by both carotid artery ligation and carotid artery ligation, followed by taking 3 mL of blood from the common carotid artery. Signals from (DETC)2-Fe2+-NO and Cu(DETC)2 complexes are recorded by EPR spectroscopy. A significant decrease in NO production in the hippocampus (on average 28
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Key words
electron paramagnetic resonance,spin trap,nitric oxide,copper,hippocampus,ischemia,hypoxia
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