Development of hemorrhagic fever with renal syndrome (HFRS) nasal mucosa immunization aerosol vaccine

The invention relates to a hemorrhagic fever with renal syndrome (HFRS) nose drop or aerosol vaccine. According to the invention, traditional injection is changed into nasal mucosa administration. The development is an important innovation and out-breaking of vaccine immunization approach. The innovative source of the invention is that: type I and II viruses separated from HFRS patient early acute whole blood and Rattus norvegicus are adopted as virus seed; the virus seed is successfully adapted to Vero cells, and is subjected to massive proliferation; multi-column tandem chromatography methods such as Matrex Cellufine Sulfate affinity chromatography and Sepharose4FF gel filtration are invented; and through vector screening optimization, the HFRS aerosol vaccine with the nasal mucosa administration approach is prepared. Also, a HN-2012JZQ aerosol vaccination gun is developed. According to animal tests, the vaccine has immunogenicity and immune reactivity. The invention provides important application basis theoretical accordance for large-scale production of simple and safe aerosol vaccines which are convenient to use.