Embryogenic callus induction, proliferation, protoplast isolation, and PEG induced fusion in Camellia oleifera

Camellia oleifera (C. oleifera) is one of the most important economic oil species in China. This study established an efficient protocol for the induction and multiplication of embryogenic callus, and the isolation, purification and Polyethylene glycol (PEG) induced fusion of protoplasts in C. oleifera. It is shown that the embryogenic callus induction was best when nodular embryogenic structures were treated with the hormone of 1.5 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) and 1.5 mg/L 6-Benzylaminopurine (6-BA) on Murashige and Skoog (MS) medium, and the highest induction rate of embryogenic callus was 18.82