Agrobacterium and biolistic mediated genetic transformation of mungbean cultivar Samrat using embryogenic explant

Mungbean, a socioeconomically important legume, is a key player in the pursuit of food security and sustainable agriculture demanding attention for developing a highly efficient transformation system to support functional genomics and translational research. This study was focused to fill in the gap using both Agrobacterium-mediated and biolistic methods by experimenting with variables of the procedure to attain higher regeneration and transformation efficiencies. The study found comparable regeneration and transformation efficiencies between direct and indirect methods of gene transfer under selection pressure. Biolistic transformation of embryonic axis explants yielded an average transformation efficiency of 14.22