Figure 6 from Shortwave Infrared Imaging Enables High-Contrast Fluorescence-Guided Surgery in Neuroblastoma

Imaging of stained cell pellets beneath tissue-mimicking material using multispectral NIR-I/SWIR fluorescence imaging. A, Microcentrifuges tubes containing pellets of 2 × 106 GD2-positive cells (LAN-1) and GD2-negative cells (SUPT1-WT) stained with 100 nmol/L of anti–GD2-IR800 and covered with a 2% emulsion intralipid. The pellet is located at the tip of the tube. The fluid level is marked in red on the Petri dish. B and C, Multispectral NIR-I/SWIR fluorescence images were captured. Images show the anti–GD2-IR800–stained GD2-positive cells (LAN-1; B) and the anti–GD2-IR800–stained GD2-negative cells (SUPT1-WT; C). White dotted lines show the approximate location of the microcentrifuge tube. D, Line profiles across the fluorescence images were fitted with a Gaussian plus linear background to extract a height and FWHM. Fits shown as a black line. E–G, Bar graphs of the height (E and F) and the FWHM (G and H) versus depth for each wavelength band for GD2-positive (LAN-1) cells stained with anti–GD2-IR800 (E and G) and anti–GD2-IR12 (F and H).