Supplementary Figure 1 from Lurbinectedin Specifically Triggers the Degradation of Phosphorylated RNA Polymerase II and the Formation of DNA Breaks in Cancer Cells

Figure S1. Structures of (A) PM030779 and (B) PM120306; both compounds are analogues of lurbinectedin. (C) Antiproliferative activity of PM120306 in A549 lung cancer cell line. Cell viability was analysed 72 h post lurbinectedin treatment by MTT assay. IC50 value was determined as being 49.9 nM (D) Western blot analyses of A549 cell extracts after (1h and 4h) treatment with either lurbinectedin (Lur, 30nM) or the biotinylated analog (PM120306, 30nM); NT: non-treated cells. The same antibody has been used to detect the hyper-phosphorylated (IIo) and hypo-phosphorylated (IIa) forms of the Rpb1 subunit of Pol II; β-Tubulin: Tub.