Supplementary Figure 2 from Myeloid Cells Obtained from the Blood but Not from the Tumor Can Suppress T-cell Proliferation in Patients with Melanoma

PDF file, 12K, Peripheral blood myeloid cells from healthy donors (n=2) were stained and sorted and their suppressive activity was tested in a co-culture with autologous T cells activated in presence of anti-CD3/anti-CD28 beads in duplicates. T cell proliferation was assessed by quantifying the percent of T cells that had not diluted the CFSE at day 4 and calculating the percent inhibition of T cell proliferation. Average percent inhibition � SEM is shown.