FIGURE 3 from Exhausted Tumor-infiltrating CD39⁺CD103⁺ CD8⁺ T Cells Unveil Potential for Increased Survival in Human Pancreatic Cancer

Expression of coinhibitory markers on T cells expressing chemokine receptors. A, Representative flow cytometry zebra plots on CD8+ T cells showing the gating strategy to identify CCR5, CXCR3, CXCR4, CXCR5, and CXCR6 positive cells. B, Proportion of CCR5 (n = 12), CXCR3 (n = 15), CXCR4 (n = 14), CXCR5 (n = 12), and CXCR6 (n = 6) on CD4+ and CD8+ T cells from central and peripheral tumor tissues, non-tumor tissues and patient PBMCs. C, Expression of PD-1, TIM-3, and PD-1-TIM-3 coexpression on CD4+ and CD8+ T cells expressing (+) or not (−) CCR5, CXCR3, CXCR4, CXCR5, and CXCR6 in central and peripheral tumor tissues of 6–7 patients. B, Friedman test followed by Dunn test was used to evaluate significant difference between groups. C, Wilcoxon matched pairs signed-rank test was used to detect statistically significant differences. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.