Supplementary Figure 2 from Loss of Keratinocytic RXRα Combined with Activated CDK4 or Oncogenic NRAS Generates UVB-Induced Melanomas via Loss of p53 and PTEN in the Tumor Microenvironment

Supplementary Figure S2: Chronic-UVB treated and parallel non-treated controls used in this study. (A,B) H&E staining of non-UVB treated skin from parallel age-matched mice (A) and TAN skin from chronic-UVB treated mice (B). In all groups, TAN skin has similar morphology to non-UVB treated skin, except for increased epidermal thickening; and dermal pigmentation which is enhanced further in the RXRaep-/- mice. Mice carrying Cdk4R24c in all groups show sebaceous gland hyperplasia within hair follicles (yellow boxes). E=Epidermis, D=Dermis, scale bar =100 IJm. (C) Epidermis measurements taken from H&E stained samples. In non-UVB treated mice, Tyr-NRAS0 61K or Cdk4R24c combined with Rxraep-/- mutation exhibited slightly thicker epidermis than RxraL2/L2 counterparts [7]. This is attenuated by chronic-UVB treatment, which results in a similarly thickened epidermis across all groups. ** = p<0.1 , ***= p