Supplementary Figures 7 and 8 from MAP17 (PDZKIP1) Expression Determines Sensitivity to the Proteasomal Inhibitor Bortezomib by Preventing Cytoprotective Autophagy and NFκB Activation in Breast Cancer

Supplementary Figure S7 - MAP17 levels determine the sensitivity to bortezomib regulating autophagy. Cells were treated with the indicated drug concentrations and performed western blot as described (see Supplementary Methods). (A) levels of proteins of the autophagosomal markers LC3B (LC3-II), in T47D and MDA-MB-231 cells overexpressing MAP17 or expressing only empty vector. (B) Quantification of proteins from A, measured as the fold increase (from control untreated cells) of protein referred to a-tubulin. Supplementary Figure S8 - MAP17 overrexpression increases endogenous ROS. Quantification of intracellular ROS leves in T47D and MDA-MB-231 cell lines. Average and SD of three independent experiments are shown. ***, P<0.001 (Student's t-test).