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Thermostable vacuum foam dried Newcastle disease vaccine: Process optimization and pilot-scale study

Fang Lyu,Yan-hong Zhao,Xiao-xin Zuo, Babalwa Nyide,Bi-hua Deng,Ming-xu Zhou,Jibo Hou, Jia-jie Jiao,Min-qian Zeng, Hong-ying Jie,Ademola Olaniran,Yu Lu,Thandeka Khoza

Applied Microbiology and Biotechnology（2024）

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Abstract
Vacuum foam drying (VFD) has been shown to improve the thermostability and long-term shelf life of Newcastle Disease Virus (NDV). This study optimized the VFD process to improve the shelf life of NDV at laboratory-scale and then tested the optimized conditions at pilot-scale. The optimal NDV to T5 formulation ratio was determined to be 1:1 or 3:2. Using the 1:1 virus to formulation ratio, the optimal filling volumes were determined to be 13–17
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Key words
Vacuum foam drying,Process upscaling,NDV vaccine,Thermostability
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