Figure 2 from PIGA Mutations and Glycosylphosphatidylinositol Anchor Dysregulation in Polyposis-Associated Duodenal Tumorigenesis

Flow cytometry analysis of FLAER positive cells in 11 FAP duodenal organoids with and without somatic PIGA variants. Fluorescence from FLAER is detected using the FITC-A channel (emission:475–650 nm). A, Abundance of FLAER positive cells detected in organoid lines with and without PIGA somatic mutations demonstrating lack of FLAER positive cells in PIGA mutant lines, indicating loss of GPI-anchors (Supplementary Table S2). B, Representative histogram of FITC-A/FLAER (selecting for FLAER positive cells), light grey = wild-type adenoma, dark grey = PIGA mutant adenoma. Repeats are denoted with a black triangle, circle, and square.