CCR2 silencing in sensory neurons blocks bone cancer progression

The peripheral nervous system has been shown to contribute to cancer growth by expanding the immunological niche. How the nervous system affects bone cancer progression and how neuroimmune pathways can be targeted for cancer treatment are not yet clear. Here, we demonstrate a profound influence of the peripheral nervous system on tumor progression, which can be targeted by silencing neuronal chemokine receptor signaling. We show that axotomy in animals with bone cancer inhibits tumor progression. Conversely, intrathecal injection of a known tumor-associated proinflammatory chemokine, CCL2, promotes tumor growth and allodynia. Silencing CCR2 in DRG neurons through a newly developed gene therapy successfully impedes tumor progression and bone remodeling and relieves bone cancer-associated pain. We demonstrate that the mechanism underlying CCR2-mediated tumor progression involves decreased neuropeptide secretion by peripheral nerves that promote expansion of the tumor-associated macrophage population. Silencing the CCR2 receptor in DRG neurons successfully normalizes the neuropeptide milieu and ameliorates altered bone remodeling. Thus, we have developed a novel therapeutic pathway for targeting a neuroimmune axis that contributes to cancer progression. ### Competing Interest Statement The authors have declared no competing interest.