The Enzyme Histochemistry of Eimeria-Infected Lambs

Objective: We aimed to investigate the effects of Eimeria infection on ANAE-positive lymphocyte percentages of lambs in the present study. Materials and Methods: For this purpose, fecal samples were taken from 200 animals into sterile containers on day 0. Eimeria positivity was detected in lambs according to the Fulleborn saturated saline method. Besides, oocyst load was detected by using the Mc. Master method in lambs. Forty of 200 lambs (n=40) aged approximately 60-75 days from a farm where Merino sheep were raised were included in this study. Experimental groups were established as Eimeria-positive male lambs (n =10), Eimeria-negative male lambs (n =10), Eimeria-positive female lambs (n =10), Eimeria-negative female lambs (n =10). Blood samples were collected to determine ANAE-positive lymphocyte percentages in lambs. Results: Results of the present study shown that Eimeria positivity was detected in 10 male and 10 female lambs according to the Fulleborn saturated saline method. According to the Mc. Master method, oocyst load was determined between 10.000-15.000 in male and female positive lambs. Eimeria-positive male and female lambs had the greatest ANAE-positive lymphocyte percentages in our study (p < 0.05). Conversely, the lowest ANAE-positive lymphocyte percentages were found in Eimeria-negative female lambs, which were not statistically different from male lambs in the present study. It was the first report according to the detection of ANAE-positive lymphocyte percentages in Eimeria-infected lambs. Conclusion: In conclusion, ANAE-positive lymphocyte percentages can be affected by Eimeria positivity and oocyst load (between 10.000-15.000) both in male and also female lambs.