Cadmium Effects on Superoxide Dismutase Activity in Litopenaeus vannamei Hemolymph
Thalassas: An International Journal of Marine Sciences(2024)
Abstract
Under controlled laboratory conditions the Cd effects on hemolymph superoxide dismutase (SOD) activity were evaluated at 8.8, 24.9 and 249 µg Cd/L and exposure times of 5, 48, and 96 h. Shrimp hemolymph samples were extracted from the first abdominal segment and hemocytes separated by centrifugation, and SOD activity (units/mg of protein in shrimp hemolymph) was quantified using the RANSOD assay kit. No mortality was observed in the controls and treatments. Superoxide dismutase activity ranged from 0.938 ± 0.0938 to 0.946 ± 0.042 SOD units/mg of protein in shrimp hemolymph in controls. At 5-h exposure, SOD activity decreased at all Cd concentrations, with significant differences (p < 0.05) than those of controls. However, a significant (p < 0.05) SOD activity increase was observed at 48-h exposure in those shrimp exposed to 24.9 and 249 µg Cd/L; but a significant (p < 0.05) SOD decrease was determined in those specimens exposed to highest Cd levels at 96-h exposure. More experiments such as those in this study are required for future updates of protection criteria for Cd in estuarine and marine environments.
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Key words
Metal,Oxidative Stress,Crustaceans,SOD
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