Assessing nutritional quality and gas production kinetics: incorporating Tithonia diversifolia into sugarcane silage

The use of unconventional feedstuffs aims to incorporate alternative dietary options to contribute to the sustainability of livestock systems. The purpose of this study was to investigate the impact of incorporating Tithonia diversifolia (TD) into sugarcane (SC) silage on nutritional quality and ruminal fermentation in an in vitro gas production assay. The underlying hypothesis was that the increased protein content in silage with the addition of TD may result in a reduction in total gas production, including methane, due to potential modifications in the substrate fermentation profile. Treatments were formulated based on the inclusion of TD in SC silage, on a dry matter basis, as follows: TC: 100