Analysis and Biological Assessment of Silver Nanoparticles Obtained from Methanol Extract of Piper Cubeba’s Fruit
Waste and Biomass Valorization(2024)
Abstract
The methanol fruit extract of Piper cubeba was used to create the silver nanoparticles (Ag-NPs). Then, their capacity to stop the growth of two Gram-positive bacteria, Bacillus cereus (6 mm) and Staphylococcus aureus (5 mm), as well as two Gram-negative bacteria, Salmonella typhi (3 mm) and Escherichia coli (5 mm), was tested. Also measured the zone of inhibition for Aspergillus Sp. and Candida spp. Fungus. By using the UV-visible spectrum (440 nm absorbance value), FT-IR (which demonstrated the existence of the O-H and C-N group), Powder-XRD revealed the peaks at 32.3o, 38.01o, 46.3o and 67.7o. By using SEM with EDAX, the nature of (Ag-NPs) could be studied, and it was found that Ag-NPs often had a spherical form and is average size is 90.3 nm with a range of 70–110 nm. The antioxidant activity of synthesized Ag-NPs was evaluated at different doses employing the Scavenging Assay, DPPH technique, and an Incubation concentration of 50
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Key words
Piper cubeba,Antimicrobial,Antioxidant,Cell line
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