Identification of a novel Providencia species showing multi-drug-resistant in three patients with hospital-acquired infection
International Journal of Antimicrobial Agents(2024)
Abstract
Providencia species are important opportunistic pathogens for humans and are associated with several infectious diseases. In this study, we found three clinical strains belonging to a novel Providencia species, namely Providencia huashanensis, including strains CRE-3FA-0001T, CRE-138-0026, and CRE-138-0111. These strains were recovered from three patients, and all of them were associated with nosocomial infections, including surgical site, urinary tract, and intracranial infections. The three strains showed high-level resistance to many types of antimicrobials, including amikacin, aztreonam, ceftazidime, cefepime, ciprofloxacin, colistin, polymyxin B, imipenem, meropenem, ceftazidime-avibactam, imipenem-relebactam. Investigation of the resistance mechanism revealed that acquired resistance genes such as blaKPC, blaNDM, blaPER, blaOXA, aac, ant, qnrD, etc., played an important role in the multidrug-resistant phenotype for the three strains. The phylogenetic trees were reconstructed based on the 16S rRNA gene sequences, multi-locus sequence analysis, and core SNPs. The genome sequence of the strains had a range of 83.5-85.8% average nucleotide identity (ANI) and 21-25.5% in silico DNA-DNA hybridization (isDDH) score with other Providencia type strains. The ANI&isDDH values and the phylogenetic tree indicated that the strains CRE-3FA-0001T, CRE-138-0026, and CRE-138-0111 strains should be considered as a novel species of the genus Providencia, for which the name P. huashanensis sp. nov. is proposed. The type strain is CRE-3FA-0001T =CCTCC AB 2023186T=KCTC 8373T.
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Key words
Providencia huashanensis,Multidrug resistance,Phylogenetic tree,hospital-acquired infections,novel species
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