Exploring the Antiproliferative Potential of Morpholine-Functionalized Aurones: Design, Synthesis, SAR, DFT, Hirshfeld Surface, 3D Energy Frameworks and Molecular Docking Analysis

In quest of generating a diverse array of potential cytotoxic candidates through molecular hybridization approach, herein we have designed a library of forty-one morpholine-functionalized aurones 7(a-ao), each with a distinct substitution pattern. These aurones were obtained by reacting 6-hydroxyaurones, with 4-(2-chloroethyl)morpholine hydrochloride, characterized by various spectroscopic techniques including single crystal XRD (7b) and in-vitro screened for their antiproliferative potential against human gastric adenocarcinoma (AGS) cells. Most of the synthesized compounds have exhibited excellent cytotoxic results. Twenty analogs were found to have cytotoxic potential better than the reference drug Leucovorin and analog 7j emerged as the most potent with IC50=5.98 mu M nearly one fifth to the standard drug Leucovorin (IC50=30.8 mu M). This library, consisting of twenty three novel and eighteen reported morpholine-functionalized aurones, bearing diverse substitution pattern, further helped in making a concrete structure-activity relationship for antiproliferative potential. Additionally, in-silico molecular docking, Hirshfeld surface analysis and two dimensional fingerprint plots were also studied to understand the molecular interactions and various structural aspects. Further computational investigations of these compounds were conducted using the DFT/B3LYP method, for the determination of HOMO and LUMO energy values as well as the calculation of chemical softness, hardness, electronegativity and electrophilic index etc. We successfully designed and synthesized a library of forty one 7(a-ao) diversely functionalized morpholine tethered aurone analogs. All the synthesized hybrids were also screened for their antiproliferative potential against human gastric adenocarcinoma (AGS) cells. Twenty of the synthesized compounds exhibited IC50 values even lower than the standard drug Leucovorin and the IC50 for analog 7 j (IC50=5.98 mu M) was nearly one fifth to the standard drug Leucovorin (IC50=30.8 mu M). image