Adhesion-driven vesicle translocation through membrane-covered pores

Translocation across barriers and through constrictions is a mechanism that is often used in vivo for transporting material between compartments. A specific example is apicomplexan parasites invading host cells through the tight junction that acts as a pore, and a similar barrier crossing is involved in drug delivery using lipid vesicles on the skin. Here, we use triangulated membranes and energy minimization to study the translocation of vesicles through pores with fixed radii. The vesicles bind to a lipid bilayer spanning the pore, and the adhesion-energy gain drives the translocation; the vesicle deformation while squeezing through leads to an energy barrier. In addition, the deformation-energy cost for deforming the pore-spanning membrane hinders translocation. Increasing the bending rigidity of the pore-spanning membrane and decreasing the pore size both shift the barrier’s maximum to smaller translocation fractions. We compare the translocation of initially spherical vesicles with fixed membrane area and freely adjustable volume with that of initially prolate vesicles with fixed membrane area and volume. In the latter case, translocation is entirely suppressed. Our predictions may help rationalize the invasion of apicomplexan parasites into host cells and design measures to combat the diseases they transmit. ### Competing Interest Statement The authors have declared no competing interest.