Synthesis of 1,3‐Disubtitituted Tetrahydropyrimidinium Salts and Determination of Their Biological Properties and Molecular Docking

AbstractSeveral of 3,4,5,6‐tetrahydropyrimidinium salts with 1‐methyl functionalization are produced. By using techniques for 1H‐NMR, 13C‐NMR, and IR spectroscopy, all compounds were investigated. Additionally, these compounds’ abilities to block enzymes were looked into. They had a highly effective inhibitory effect on the isoenzymes of carbonic anhydrases I and II, butyrylcholinesterase (BChE), and acetylcholinesterase (AChE). Ki values were found in the range of 57.43±7.09–170.09±50.91 nM for AChE, 7.19±0.42–69.08±2.44 nM for BChE, and 46.48±5.74–203.38±46.15 nM for hCA I, and 30.19±4.03–171.96±30.27 nM for hCA II. As a result, 1,3‐disubtitituted tetrahydroprimidinium salts exhibited potent inhibition profiles toward indicated metabolic enzymes. One of the most important methods for designing and creating novel, potent medications to treat Alzheimer's disease (AD) worldwide is the synthesis and discovery of new AChE and BChE inhibitors. The activities of synthesized 3,4,5,6‐tetrahydropyrimidinium salts were compared against various proteins that are crystal structure of AChE (PDB ID: 4 M0E), crystal structure of BChE (PDB ID: 5NN0), crystal structure of hCA I (PDB ID: 2CAB), and crystal structure of hCA II (PDB ID: 3DC3), and then the drug properties of these molecules were examined.