Figure 4 from FUME-TCRseq Enables Sensitive and Accurate Sequencing of the T-cell Receptor from Limited Input of Degraded RNA

A, Hematoxylin and eosin showing the regions of FFPE adenoma and carcinoma that were macrodissected for RNA extraction and FUME-TCRseq. Scale bar, 5 mm. B, Bar chart showing the numbers of unique TCR clonotypes detected in carcinoma and adenoma regions. C, Bar chart showing the diversity of the TCR repertoire in carcinoma and adenoma regions. D, Plot showing the frequencies of the 10 most common clonotypes detected in the adenoma region and the frequencies at which they appear in the carcinoma.