A rapid solid-phase extraction purification combined with UPLCMS/MS used for simultaneous determination of eight tetracyclines and three metabolite in chicken and pork

A method for rapid detection and identification of 8 tetracyclines (TCs) and 3 metabolite residues in chicken and pork was established and verified by combining rapid solid-phase extraction (SPE) technology with ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLCMS/MS). After a sample was extracted, it was purified with a PRiME HLB cartridge, and the cartridge did not need to be activated, balanced, or eluted. The filtrate was dried under nitrogen, redissolved, and subsequently analyzed via UPLCMS/MS. The limits of detection for chicken and pork were 0.05–0.48μg/kg and 0.05–0.52μg/kg, respectively. The limits of quantification were 0.16–1.61μg/kg and 0.16–1.75μg/kg, respectively. The recoveries were 85.15%–107.50% and 84.21%–106.37%, respectively. The established method was suitable for qualitative and quantitative analyses of TCs and their metabolite residues in chicken and pork and has been used for the detection of TC in chicken and pork samples from local markets.