Si-rhodamine derivative with a large stokes shift for ELISA-based detection of SARS-CoV-2

In view of the worldwide impact of SARS-CoV-2, developing rapid and accurate ELISA-based methods for detecting SARS-CoV-2 is of great importance for diagnosing and controlling coronavirus disease 2019. Herein, we report highly stable and fluorescently bright Si-rhodamine analogues with obviously improved Stokes shifts for IgG antibody labelling. These new fluorescent dye labels provide a promising fluorescence tool for SARS-Cov-2 detection. An Si-rhodamine derivative with a large Stokes shift and high fluorescence brightness was applied to distinguish normal serum from serum of COVID-19 patients.