Blood cortisol concentration in response to recurrent manipulation in crossbred Holstein embryo donors
REVISTA CIENTIFICA-FACULTAD DE CIENCIAS VETERINARIAS（2024）
摘要
The neuro-endocrine response of the donor was determined by reducing the number of applications of follicle-stimulating hormone (FSH) as a superovulation factor. Eight crossbred Holstein cows were randomly distributed into two treatments (T): T1 (n=4) received a dose of 200 mg of FSH via epidural (EP) on day 4 of the superovulation protocol (SOV), in T2 300 were administered. mg of FSH divided into 8 decreasing doses intramuscularly (IM), on days 4; 5; 6 and 7 of the protocol in the morning and afternoon. Cortisol concentration was determined at 0; 2; 4; 6,24; 26; 28; 30; 48; fifty; 52; 64; 72; 74; 76 and 78 hours (h) of day 4. Heart rate (HR), respiratory rate (RR) and glucose levels were evaluated on day 4 at 05h45; 06h05; 08h00; 10h00; 12h00; 5:45 p.m. and 6:05 p.m. Cortisol at 0h was similar in the two Ts: T1 (10.9 +/- 0.24 mu gdL (-1) ) and T2 (10.8 +/- 0.33 mu gdL (-1) ) and reached its highest level at 0h 4h in T1 (15.8 +/- 1.39 mu gdL (-1) ) and in T2 (16.4 +/- 0.28 mu gdL (-1) ) with no difference between T ( P >0.05). In the following assessments, cortisol decreased to basal levels in both Ts. HR at 05h45 showed similar values in T1 (65.3 +/- 1.65 pulse min (-1) ) and in T2 (66.3 +/- 1 .43 inmin (-1) ); However, at 06h05 HR increased in T1 (74.3 +/- 1.54 inmin (-1) ) and in T2 (84.2 +/- 1.73 inmin (-1) ) and decreased from 08h00. The same behavior was observed with RR and glucose. It is concluded that taking the donor to the holding sleeve to administer FSH constitutes a stressor that increases HR, RR, cortisol and glucose, factors that influence the percentage of ovulations in the two T.
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关键词
Cortisol,epidural,donors,Holstein,superovulation
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