Syringic Acid Attenuates the IL-1-Induced Akt Pathway in Chondrocyte ATDC5 Cells

Background Osteoarthritis (OA) is a chronic progressive joint ailment that is largely predominant worldwide. However, it typically gets worse over time, occurs more frequently, and becomes more crippling.Objectives Syringic acid (SA) is a well-known phenolic compound reported to suppress inflammation, cell proliferation, and apoptosis of various cancer cells. Since the role of SA in OA remains unknown, there is a need to hypothesize the anti-inflammatory activities of SA on IL-1 beta-induced ATDC5 chondrocyte-like cells and to elucidate its protective action against OA.Methods The cytotoxicity, inflammatory mediators, mRNA expression of MMPs, ADAMTS, COX-2, and Akt/NF-kappa B protein expression of SA activity on ATDC5 cells were examined through CCK-8 assay, ELISA, RT-qPCR, and western blot. It was found that SA (10, 20, and 30 mu M) did not show any inhibitory effects on the viability of the ATDC5 cells in a concentration dependent manner.Results SA markedly reduced the inflammatory mediators, cytokines, PGE2, MMPs, COX-2, and ADAMTS in a concentration-dependent manner. Likewise, SA expressively attenuated IL-1 beta-stimulated Akt phosphorylation and NF-kappa B activation as well as IL-1 beta- induced ATDC5 chondrocytes.Conclusion This study revealed that SA is a novel candidate applicable for the treatment of OA.