Research progress in the development of 3D skin models and their application to in vitro skin irritation testing

Toxicological assessment of chemicals is crucial for safeguarding human health and the environment. However, traditional animal experiments are associated with ethical, technical, and predictive limitations in assessing the toxicity of chemicals to the skin. With the recent development of bioengineering and tissue engineering, three-dimensional (3D) skin models have been commonly used as an alternative for toxicological studies. The skin consists of the subcutaneous, dermis, and epidermis. All these layers have crucial functions such as physical and biological protection and thermoregulation. The epidermis is the shallowest layer protecting against external substances and media. Because the skin is the first contact point for many substances, this organ is very significant for assessing local toxicity following skin exposure. According to the classification of the United Nations Global Harmonized System, skin irritation is a major potentially hazardous characteristic of chemicals, and this characteristic must be accurately assessed and classified for enhancing chemical safety management and preventing and reducing chemical accidents. This review discusses the research progress of 3D skin models and introduces their application in assessing chemical skin irritation. Assessing chemical toxicity is essential for maintaining the safety of the human health and environment. Traditional animal experiments face ethical, technical, and predictive limitations, the application of alternative models becomes critical. This review focuses on and summarizes the research progress of 3D skin models and their application in the assessment of chemical-induced skin irritation, providing a basis for the development of in vitro models and their application to skin irritation.