Supernatant fluid from endobronchial ultrasound-guided transbronchial needle aspiration for rapid next-generation sequencing
Journal of the American Society of Cytopathology(2024)
Abstract
Introduction
There is an increasing demand to optimize the workflow and maximize tissue available for next-generation sequencing (NGS) for non-small cell carcinoma. We looked at transbronchial needle endobronchial ultrasound-guided bronchoscopy aspiration (EBUS-TBNA) samples and evaluated the performance of supernatant fluid (SN) processed from a dedicated aspirate collected for NGS testing.
Methods
Nineteen samples were collected and processed using a new workflow. Five aspirates were collected in formalin. One additional dedicated pass was collected fresh and centrifuged. The resulting cell pellet was added to formalin for cell block processing. DNA and RNA were extracted from concentrated SN for targeted testing using the Oncomine™ Precision Assay (Thermo Scientific™, Waltham, MA). NGS results from the corresponding cell block samples were used as “controls” for comparison.
Results
31 mutations were detected in SN (Table 1). The most frequently mutated genes were TP53 (35%), EGFR (23%), KRAS (13%), CTNNB1 (6%), and ERBB2 (6%). There was 100% concordance between the mutations detected in SN and corresponding cell blocks with comparable variant allele frequencies. TAT of NGS results was 1 day for SN compared to 4 to 10 days for cell block.
Conclusions
We were able to demonstrate the usefulness of SN for reliable, rapid molecular results. We successfully incorporated the workflow for tissue handling and processing among our clinical, cytopathology, and molecular teams. Molecular results were available at the same time as the cytologic diagnosis, allowing for timely reporting of a comprehensive diagnosis. This approach is particularly useful in patients with advanced disease requiring urgent management.
MoreTranslated text
Key words
Supernatant,EBUS-TBNA,next-generation sequencing
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined