Antimicrobial Peptide-Modified Liquid Metal Nanomaterials for Enhanced Antibacterial Photothermal Therapy

The misuse of antibiotics has led to antibiotic-resistant "superbugs," prompting the exploration of alternative antibacterial strategies. This study focuses on the promising avenue of photothermal therapy (PTT). Despite numerous advantages, the clinical applicability of PTT as a sole sterilization strategy is hindered by the necessity for higher temperatures, potentially causing harm to healthy tissues. To overcome this challenge, this study introduces antimicrobial peptides (AMPs) to modify the surface of gallium-based liquid metal (LM) nano-antimicrobial agents, thereby enhancing their photothermal antibacterial effects within a lower temperature range. First, a novel LM composite nanomaterial, LM@AMP nanoparticles, is synthesized through a sonication process involving 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)], AMP, and LM in an aqueous solution. Herein, AMPs not only contribute to the structural stability of LM nanoparticles but also enhance their selective interaction with bacterial cell membranes. Then, a thorough characterization of LM@AMP nanoparticles is performed, encompassing analyses through electron microscopy, determination of particle size, and assessment of zeta potential. Moreover, the exceptional photothermal properties exhibited by these nanoparticles are validated. Finally, this investigation demonstrates that LM@AMP nanoparticles selectively target bacterial cell membranes, showcasing efficient bactericidal effects (near 100%) at relatively low temperatures under near-infrared irradiation. Antibiotic misuse drives exploration of alternative antibacterial strategies like photothermal therapy. To mitigate damage to healthy tissues at high temperatures, liquid metal nano-antimicrobial agents with antimicrobial peptides are modified, which exhibit exceptional photothermal properties, selectively targeting bacterial cell membranes, and efficient bactericidal effects (near 100%) at lower temperatures. This innovative approach shows promise in addressing bacterial infections.image (c) 2024 WILEY-VCH GmbH