Microwave-assisted and methanol/acetic acid-free method for rapid staining of proteins in SDS-PAGE gels
Analytical Biochemistry(2024)
Abstract
We describe a microwave-assisted, methanol and acetic acid-free, inexpensive method for rapid staining of SDS-PAGE proteins. Only citric acid, benzoic acid, and Coomassie brilliant blue G-250 (CBG) were used. Microwave irradiation reduced the detection duration, and proteins in a clear background were visualized within 30 min of destaining, after 2 min of fixing and 12 min of staining. By using this protocol, comparable band intensities were obtained to the conventional methanol/acetic acid method.
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Key words
CBG stain,methanol/acetic acid-free,microwave-assisted,Coomassie brilliant blue G-250,citric acid
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