Sensitive detection of exosomes by an electrochemical aptasensor based on double nucleic acid amplifications

Microchemical Journal(2024)

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摘要
Exosomes are extracellular vesicles secreted by mammalian cells and recognized as a powerful non-invasive cancer marker because they carry the cellular components specific to parent cells. The development of a convenient, rapid and sensitive exosome detection method is very important for the early diagnosis of cancer. In this study, an electrochemical biosensor based on two signal amplification methods, primer exchange reaction (PER) and rolling circle amplification (RCA), was developed for the high sensitive detection of exosomes. First, exosomes were specifically captured by CD63 aptamer and DNA strands (T) were released through a chain replacement reaction. Then the T strands initiated the PER reaction, producing a large number of short strands of DNA (P). Afterwards, RCA reaction was carried out on the electrode surface. Finally, a large amount of horseradish peroxidase (HRP) was bound to the electrode surface to catalyze o-phenylenediamine (OPD) oxidation, and the reduction current signal was obtained to realize the quantitative detection of exosomes. Under the optimal experimental conditions, there was a good linear relationship between DPV signal and the logarithm of exosome concentration in the range of 88 ∼ 8.8 × 106 particle/μL with a low limit of detection of 22 particle/μL. This strategy could realize sensitive detection of exosomes and provide a new strategy for the detection of other tumor markers.
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关键词
Exosomes,Electrochemical aptasensor,Primer exchange reaction,Rolling circle amplification
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