Self-Healing Efficiency of Later-Age Cracked Microbial Mortar Embedded with Unencapsulated Microorganisms under Long-Term Water and Ambient Exposure Conditions

To ensure the long-term self-healing ability of concrete structures throughout their lifespan is a crucial requirement for Microbially Induced Carbonate Precipitation (MICP) technology. In this study, unencapsulated microbial cells were directly mixed into the fresh mortar as a component to investigate their efficiency in repairing later-age cracks, respectively exposed to long-term water environment and ambient conditions. The crack width repair ratio for samples with cracks initially created by splitting tests was calculated at healing intervals of 6 and 12 days, revealing a sixfold higher repair ratio in 150-day samples with initial widths below 200 μm than those cases with 500 μm-wide cracks. The 26.3% reduction in water absorption content after a 12-day healing period reflected an effective healing result for cracks and capillary pores. The microbially induced calcite precipitations were confirmed through SEM, XRD, and EDS analysis, with complete crack closure observed for 150-day samples with widths less than 200 μm. However, it was also found that the self-healing efficiency decreased with increasing crack width due to the limited availability of healing agents, with the maximum completely healed crack width of 507.8 μm. Despite prolonged exposure, no further reduction in crack width and sorptivity was observed, highlighting the challenges in achieving a complete healing effect. Surface images obtained from microscopes revealed the enduring presence of unencapsulated microorganisms, corroborating their role in continuous calcite precipitation and crack filling.