Green Synthesis of Silver doped Zinc Oxide/Magnesium Oxide Nanocomposite for Waste Water Treatment and Examination of their Cytotoxicity Properties

This research attempted to prepare silver-doped zinc oxide/magnesium oxide nanocomposite (Ag-doped ZnO/MgO-NCP) using Mentha pulegium plant extract. The synthesized NCP was investigated by X-ray diffraction analysis (XRD), Fourier Transform Infrared (FT-IR), Field Emission Scanning Electron Microscope (FESEM), Energy dispersive X-ray spectroscopy (EDX), Mapping, and UV-Visible analyses. The XRD data displayed cubic crystal structures for silver & magnesium oxide and a hexagonal framework for zinc oxide. Also, FESEM and PSA images of NCP pointed out, that the average size of the spherical morphology is about 10 to 16 nm, while the analysis of EDX confirmed the attendance of Zn, Mg, Ag, and O elements. Under UVA light, we tested the photocatalytic activity of NCP to the degradation of Methylene blue (MB) and Rhodamine B (RhB) dyes in various temperatures (400, 500, and 600 °C). The results of the photocatalytic test displayed that the degradation percentage of MB dye in pH = 9, nanocomposite amount ∼ 30 mg, and dye concentration ∼ 1×10 -5 M was about 98%. We also evaluated the cytotoxicity of nanocomposite on cancer CT-26 cell line through the MTT method and obtained an IC50 value of 250 μg/mL.