Whole genomes show contrasting trends of population size changes and genomic diversity for an Amazonian endemic passerine over the late quaternary

The "Amazon tipping point" is a global change scenario resulting in replacement of upland terra-firme forests by large-scale "savannization" of mostly southern and eastern Amazon. Reduced rainfall accompanying the Last Glacial Maximum (LGM) has been proposed to have acted as such a tipping point in the past, with the prediction that terra-firme inhabiting species should have experienced reductions in population size as drier habitats expanded. Here, we use whole-genomes of an Amazonian endemic organism (Scale-backed antbirds - Willisornis spp.) sampled from nine populations across the region to test this historical demography scenario. Populations from southeastern Amazonia and close to the Amazon-Cerrado ecotone exhibited a wide range of demographic patterns, while most of those from northern and western Amazonia experienced uniform expansions between 400 kya and 80-60 kya, with gradual declines toward 20 kya. Southeastern populations of Willisornis were the last to diversify and showed smaller heterozygosity and higher runs of homozygosity values than western and northern populations. These patterns support historical population declines throughout the Amazon that affected more strongly lineages in the southern and eastern areas, where historical "tipping point" conditions existed due to the widespread replacement of humid forest by drier and open vegetation during the LGM. Here, we make a pioneering effort to use whole-genomes to test for the effects of former episodes of large-scale "savannization" of the Amazon. Our findings support historical population declines throughout the Amazon for an endemic bird, which were stronger in populations inhabiting southern and eastern Amazonia, where widespread replacement of humid forest by open vegetation during the Last Glacial Maximum has been inferred by multiple studies. These results highlight the historical resilience of Amazonian biological communities which have persisted in drier, more seasonal, and historically less stable climates, conditions expected to expand over most of the biome this century and afterward.image