Creating a zero amylose barley with high soluble sugar content by genome editing
Plant Molecular Biology(2024)
摘要
Amylose biosynthesis is strictly associated with granule-bound starch synthase I (GBSSI) encoded by the Waxy gene. Mutagenesis of single bases in the Waxy gene, which induced by CRISPR/Cas9 genome editing, caused absence of intact GBSSI protein in grain of the edited line. The amylose and amylopectin contents of waxy mutants were zero and 31.73
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关键词
Barley,Starch,Waxy,Amylose-free,CRISPR/Cas9 genome editing
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