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CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations

Xiao-Wen Fan,Zi-Fan Gao,Dong-Dong Ling, De-Hui Wang,Ying Cui,Hui-Qun Du,Chun-Lin Li,Xing Zhou

Military Medical Research（2024）

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关键词
Cas9 nickase,CRISPR-Cas12a,Polymerase,Single base mutations
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