1-Dodecanol as Potential Inducer for the FAO1 Promoter (PFAO1) in Morphologically Identified Meyerozyma guilliermondii Strain SO
Arabian Journal for Science and Engineering(2024)
Abstract
Alcohol oxidase (AOX) oxidizes alcohols to produce carbonyl compounds and peroxides. Its promoter (PAOX1) is widely used in methylotrophic yeasts. A promising yeast expression system (Pichia sp. strain SO) was developed for bacterial lipase expression regulated by PAOX1 of Komagataella phaffii (previously known as Pichia pastoris). However, its unidentified AOX gene and the protein structure have deterred the search for the best inducer. This study was aimed to identify the yeast species and determine the best inducer for PAOX1 upregulation using in silico AOX protein analysis. Morphological (scanning and transmission electron microscopies) and carbon assimilation analyses confirmed isolate SO as Meyerozyma guilliermondii (previously known as Pichia guilliiermondii). Using Hidden-Markov model and degenerate PCR, the LCAO gene (2091 bp) was discovered in M. guilliermondii strain SO. The enzyme, MgFAO1 shared 14
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Key words
Long chain alcohol oxidase,Substrate specificity,Alcohol oxidase,Meyerozyma guilliermondii,Yeast expression system,Hidden Markov model
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