Rosellichalasins A–H, cytotoxic cytochalasans from the endophytic fungus Rosellinia sp. Glinf021

Eight new cytochalasans rosellichalasins A–H (1–8), as well as two new shunt metabolites rosellinins A (9) and B (10) before intramolecular Diels–Alder cycloaddition reaction in cytochalasan biosynthesis, along with nine known cytochalsans (11–19) were isolated from the endophytic fungus Rosellinia sp. Glinf021, which was derived from the medicinal plant Glycyrrhiza inflata. Their structures were characterized by extensive analysis of 1D and 2D NMR as well as HRESIMS spectra and quantum chemical ECD calculations. The cytotoxic activities of these compounds were evaluated against four human cancer cell lines including HCT116, MDA-MB-231, BGC823, and PANC-1 with IC50 values ranging from 0.5 to 58.2 μM.