FIGURE 2 from The Cross-talk Between Intestinal Microbiota and MDSCs Fuels Colitis-associated Cancer Development

CAC-bearing mice display altered microbiota composition and diversity. A, Schematic presentation of different timepoints for fecal samples collection. Created with BioRender.com. PCoA plots showing microbiota composition analysis of control (B) and CAC (C) mice at the different timepoints during the experiment. D, α diversity measured using Faith's PD. E, Heat map based on Calour, depicting changes in bacteria between the groups throughout the experiment (overrepresented bacteria are marked with red arrows). White dashed lines separate the CAC and control groups at each timepoint. F, FITC-dextran fluorescence intensity in serum normalized to control mice (n = 4–7 per group). G, FISH staining of bacteria in colonic tissue sections of CAC-bearing mice compared with control mice. White arrows indicate bacterial penetration into the tissue. Asterisks indicate the lumen; Magnification: 10x, (n = 3). Magnification: 20x, Scale bar, 50 µm. Inserts, represent a magnification of the white dashed squares. Results are represented as mean ± SEM; **, P < 0.01; ***, P < 0.001.