High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems.
Methods in molecular biology (Clifton, N.J.)(2022)
Abstract
Cell-free protein synthesis can enable the combinatorial screening of many different components and concentrations. However, manual pipetting methods are unfit to handle many cell-free reactions. Here, we describe a microfluidic method that can generate hundreds of unique submicroliter scale reactions. The method is coupled with a high yield cell-free system that can be applied for broad protein screening assays.
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Key words
Automation,Cell-free protein synthesis,Droplet printing,High throughput,Low-volume liquid handling,Microfluidic adaptive printing,Protein screening
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