Biochemical and Ultrastructural Changes in the Microalgae <i>Tisochrysis lutea</i> (Bendif et Probert) (Haptophyta) at different stages of growth in enhancement culture

In our study we investigated the growth, biochemical composition, and ultrastructure of Tisochrysis lutea microalgae in enrichment culture during 30 days experiment. The number of T. lutea cells increased throughout the experiment. We noted an increase in the size and number of lipid droplets containing fatty acids and carotenoids, including fucoxanthin, in the exponential and stationary phases of their growth. It has been established that the total content of carotenoids reaches a maximum in the stationary phase and decreases in the dying phase. During the stationary phase, exocytosis is observed in cells with the release of lipid droplets. This study demonstrates the potential of the T. lutea clone MBRU_Tiso-08 from the Marine Biobank Bioresource Collection of the NSCMB FEB RAS as a raw material for domestic biotechnology aimed at the combined extraction of carotenoids (including fucoxanthin) and lipids (including docosahexaenoic and eicosapentaenoic fatty acids).