Identification and biological characteristics of Enterococcus casseliflavus TN-47 isolated from Monochamus alternatus

With the widespread use of antibiotics, the incidence of antibiotic resistance in microorganisms has increased. Monochamus alternatus is a trunk borer of pine trees. This study aimed to investigate the in vitro antimicrobial and biological characteristics of Enterococcus casseliflavus TN- 47 (PP411196), isolated from the gastrointestinal tract of M. alternatus in Jilin Province, PR China. Among 13 isolates obtained from the insects, five were preliminarily screened for antimicrobial activity. E. casseliflavus TN- 47, which exhibited the strongest antimicrobial activity, was identified. E. casseliflavus TN- 47 possessed antimicrobial activity against Staphylococcus aureus USA300 and Salmonella enterica serovar Pullorum ATCC 19945. Furthermore, E. casseliflavus TN- 47 was sensitive to tetracyclines, penicillins (ampicillin, carbenicillin, and piperacillin), quinolones and nitrofuran antibiotics, and resistant to certain beta- lactam antibiotics (oxacillin, cefradine and cephalexin), macrolide antibiotics, sulfonamides and aminoglycosides. E. casseliflavus TN- 47 could tolerate low pH and pepsin- rich conditions in the stomach and grow in the presence of bile acids. E. casseliflavus TN- 47 retained its strong auto- aggregating ability and hydrophobicity. This strain did not exhibit any haemolytic activity. These results indicate that E. casseliflavus TN- 47 has potential as a probiotic. This study provides a theoretical foundation for the future applications of E. casseliflavus TN- 47 and its secondary metabolites in animal nutrition and feed.